Low-fat, lactose-free and leucine-enriched chocolate cow milk prototype: A preliminary study on sensorial acceptability and gastrointestinal complaints following exhaustive exercise.

BACKGROUND: Chocolate milk has gained recent scientific support as a recovery drink. However, it is known that high exercise-demand triggers gastrointestinal discomfort which continues post-exercise, thereby hindering this nutritional strategy. In addition, those who are lactose intolerant cannot benefit from a milk-based beverage. Thus, the aim of this preliminary study was to develop a low-fat, lactose-free, and leucine-enriched chocolate cow milk prototype (CML) representing nutrition-related recommendations for football players, as well as assess athletes' individual subjective outcomes for gastrointestinal complaints and sensorial acceptability in a field-based setting following strenuous team-sport physical demands. METHODS: This study followed a single group and repeated-measured design with 10 football players (23 ± 2 yrs., 74 ± 14 kg, 174 ± 5 cm) who consumed CML following a 90-min football match simulation protocol (FMP). The total CML intake to achieve 0.150 g leucine·kg [BW]·h- 1 occurred in aliquots of 50, 30 and 20% at 0-, 45- and 75-min post-FMP, respectively. Athletes were evaluated by the prevalence, the type and severity (bloating, nausea, flatulence, and gastric reflux) of gastrointestinal complaints and sensorial acceptability (overall perception, appearance, consistency, and flavour) after drinking each aliquot in a 4-h recovery period. RESULTS: The CML showed higher scores for "Product Acceptability Index" (88%) and sensorial acceptability (~ 8 in 9-point hedonic scale). Kendall's W with bootstrapped resample (95%CI) revealed agreement among respondents as "moderate" (overall perception, flavour) to "strong" (appearance, consistency) and with no significant agreement differences between rater response in the timeline analysis (0.57 up to 0.87; p > 0.05). Agresti-Caffo add-4 analysis (95% confidence interval, [95%CI]) revealed no differences in each time-point analysis versus baseline for athletes classified as having severe gastrointestinal symptoms, but confirmed concern with bloating (three athletes showed a transient response at 2-h and only one continued until 3-h; p = 0.051). CONCLUSIONS: These preliminary findings suggest that CML presents good taste and high acceptability by the sampled athletes. Thus, CML may be an alternative sport drink for immediate post-workout supplementation to overcome the energy deficit, offer co-ingested leucine, maintain palatability and adherence including lactose intolerance following a team sport-specific fatigue. TRIAL REGISTRATION: RBR-2vmpz9 , 10/12/2019, retrospectively registered.

Comparison of Physical Fitness and Anthropometrical Profiles Among Brazilian Female Soccer National Teams From U15 to Senior Categories.

ABSTRACT: Ramos, GP, Nakamura, FY, Penna, EM, Mendes, TT, Mahseredjian, F, Lima, AM, Garcia, ES, Prado, LS, and Coimbra, CC. Comparison of physical fitness and anthropometrical profiles among Brazilian female soccer national teams from U15 to senior categories. J Strength Cond Res 35(8): 2302-2308, 2021-This study aimed to compare anthropometric and physical fitness of Brazilian female national team soccer players from the U15 to senior categories, and to compare the physical performance between selected and nonselected players. Subjects included 231 athletes (U15, n = 46, U17, n = 49, U20, n = 98, and Senior, n = 38). Body mass, height, sum of skinfolds, squat jump (SJ), countermovement jump (CMJ), 20-m linear sprint, and Yo-Yo IR1 were assessed. The U15 players were shorter than all other groups (p < 0.01) and lighter than U20 players (p < 0.01). Regarding physical tests, Senior athletes presented higher SJ compared with U20, and both showed higher CMJ and SJ compared with the U15 and U17 (p < 0.05). Senior athletes were also faster than players of all other categories in 20-m sprint (p < 0.01) and covered the greatest distance in the Yo-Yo IR1 (p < 0.05). U20 were better in the Yo-Yo IR1 than the younger groups (p < 0.05). When comparing selected and nonselected players, no differences were identified in anthropometric measures (p > 0.05). However, selected players from U17, U20, and Senior teams showed better performance in Yo-Yo IR1 than nonselected ones (p < 0.05). Finally, selected senior athletes also presented higher CMJ and SJ than nonselected players (p < 0.05). These results suggest that, although there is a tendency for maintenance in anthropometric measures from the age of 15 years, there are substantial improvements in speed, lower-body power, and aerobic capacity from U20 age group. In addition, it seems that intermittent aerobic fitness contributes to the selection of players to international tournaments in national teams.

Muscle Damage-Based Recovery Strategies Can Be Supported by Predictive Capacity of Specific Global Positioning System Accelerometry Parameters Immediately a Post-Soccer Match-Load.

ABSTRACT: da Silva, CD, Machado, G, Fernandes, AA, Teoldo, I, Pimenta, EM, Marins, JCB, and Garcia, ES. Muscle damage-based recovery strategies can be supported by predictive capacity of specific global positioning system accelerometry parameters immediately after soccer match-load. J Strength Cond Res 35(5): 1410-1418, 2021-Soccer match-load can be linked to recovery kinetic markers. However, match variability hinders the magnitude of relationship between parameters of interest. Therefore, we examined the correlation between 21 global positioning system accelerometry (GPS-A) parameters and changes in serum creatine kinase (CK) concentrations, muscle soreness (MS), and perceptive recovery quality (PRQ) assessed at baseline (1 h before) and post (0 minute, 2, 4, and 24 hours) a standardized 90-minute match-simulation in 20 university players. Global positioning system accelerometry (15 Hz) data were tested as manufacturer and configurable thresholds. Four GPS-A parameters showed moderate to very large correlations with CK changes at all time points (average speed [avgSP, r = 0.75 to r = 0.84]; running symmetry foot strikes [RSfst, r = 0.53-0.63]; running series [RunS, r = 0.53-0.61]; and acceleration distance [AccD ≥ 1.5 m·s-2; r = 0.46-0.61]). Sprint count (≥2 m·s-2), AccD (≥2.5 m·s-2) and speed exertion (SpEx) had a moderate to large correlation (r = 0.46-0.56) with CK changes from 2 to 24 hours. Changes in MS at 0 minute had large correlation with avgSP (r = 0.53) and moderate with deceleration distance (≥-2 and ≥-3 m·s-2; r = 0.47, r = 0.48, respectively). The PRQ changes had moderate inverse correlation with avgSP at 0 minute (r = -0.39) and SpEx at 2 h (r = -0.69). Our results suggest that during a simulated soccer protocol with a standard workload, only the avgSP has practical application for predicting CK changes over 24 hours, allowing for a decision-making toward a postgame recovery based on previously known CK cutoff points. Global positioning system accelerometry parameters and subjective variables did not demonstrate relevant correlation.

Preliminary Validation of Mirrored Scales for Monitoring Professional Soccer Training Sessions.

We aimed to create a single subjective method to assess both internal training loads and subsequent fatigue. This new training-fatigue (dose-response) scale (TFS) was composed of two similar scales with the same properties, metrics and construction criteria. These two scales were designed to rate the perceived exertion (RPETFS) and perceived fatigue (RPFTFS) in professional soccer players. Twenty-two athletes participated to establish reliability, and 15 participated to establish validity. For reliability, the intraclass correlation coefficient (ICC) and standard error of measurement (SEM) were used. For criterion validity, the Spearman's correlation coefficient and linear regression analyses were applied. Associations between RPETFS and RPFTFS were verified by a chi square test, and a further factorial exploratory analysis was conducted. RPETFS and RPFTFS were found to be reliable (ICC 0.74 and 0.77, SEM 0.30 and 0.30, respectively) and valid. RPETFS was best explained by the internal load of the Banister training impulse (p < 0.001), while RPFTFS was best explained by the internal load of the Stagno training impulse (p < 0.001). An association was found between the scales (RPETFS and RPFTFS) in which training duration had a more substantial impact on these subjective perceptions than did training intensity (p < 0.01). RPETFS and RPFTFS scales are reliable and valid for monitoring training sessions in Brazilian professional soccer players. The simultaneous oscillations of the RPETFS and RPFTFS scores can be used by staff members to better plan weekly training programs based on dose-response ratings. Finally, training duration must be carefully controlled because it has a greater impact than intensity on subjective perceptions.

Correction: The influence of a hot environment on physiological stress responses in exercise until exhaustion.

[This corrects the article DOI: 10.1371/journal.pone.0209510.].

The influence of a hot environment on physiological stress responses in exercise until exhaustion.

Exhaustive exercise in a hot environment can impair performance. Higher epinephrine plasma levels occur during exercise in heat, indicating greater sympathetic activity. This study examined the influence of exercise in the heat on stress levels. Nine young healthy men performed a maximal progressive test on a cycle ergometer at two different environmental conditions: hot (40°C) and normal (22°C), both between 40% and 50% relative humidity. Venous blood and saliva samples were collected pre-test and post-test. Before exercise there were no significant changes in salivary biomarkers (salivary IgA: p = 0.12; α-amylase: p = 0.66; cortisol: p = 0.95; nitric oxide: p = 0.13; total proteins: p = 0.07) or blood lactate (p = 0.14) between the two thermal environments. Following exercise, there were significant increases in all variables (salivary IgA 22°C: p = 0.04, 40°C: p = 0.0002; α-amylase 22°C: p = 0.0002, 40°C: p = 0.0002; cortisol 22°C: p = 0.02, 40°C: p = 0.0002; nitric oxide 22°C: p = 0.0005, 40°C: p = 0.0003, total proteins 22°C: p<0.0001, 40°C: p<0.0001 and; blood lactate 22°C: p<0.0001, 40°C: p<0.0001) both at 22°C and 40°C. There was no significant adjustment regarding IgA levels between the two thermal environments (p = 0.74), however the levels of α-amylase (p = 0.02), cortisol (p<0.0001), nitric oxide (p = 0.02) and total proteins (p = 0.01) in saliva were higher in the hotter conditions. Blood lactate was lower under the hot environment (p = 0.01). In conclusion, enduring hot temperature intensified stressful responses elicited by exercise. This study advocates that hot temperature deteriorates exercise performance under exhaustive stress and effort conditions.

Match internal load in youth elite soccer players is period, playing position and intermittent running capacity dependent

The aims of this study were: i) to verify whether player internal load (PIL) monitored via heart rate (HR) varies with game-time and playing position; ii) whether intermittent running capacity (IRC) is related to the maintenance of within-match PIL in elite youth (U-15) soccer players. Method: Twenty-one elite soccer players (14±0.5 yrs, 172±7 cm, 63±6 kg) had their heart rate monitored (beats/min) in five matches and were tested twice for IRC (Yo-YoIR2, distance [m]) over a seven-week competitive season. Percentage of maximal heart rate (%HRmax) and time spent (TS%) in five zones (Z1[<70%]; Z2[71-85%]; Z3[86-90%]; Z4[91-95%] and Z5[>96%]) were our PIL indexes. Data from three complete games in the same position of each player were analyzed and matches halves, time intervals (T1 to T6), and playing positions (fullbacks, central defenders and forwards [N=5 each], midfielders [N=6]) were compared, and the relationship between IRC and within-match PIL was determined. Results: PIL was higher in 1st (86±3%) than in the 2nd half (84±4%; p<.001). The 2nd half had more TS% in Z1 and Z2 (p<.05). PIL in T4 was the lowest (p<.01), and in T6, it was lower than T1 and T2 (p<.01). Fullbacks and midfielders showed higher PIL and higher TS% in Z4 (p<.05) than the other positional roles. The average IRC correlated with PIL in T6 (r=.56, p<.01) only. Conclusion: In conclusion, the internal load in elite youth (U-15) soccer players varies with game-time and playing position; and their IRC is related to the maintenance of within-match PIL.(AU)

Effect of ACTN3 gene on strength and endurance in soccer players.

Sports efficiency in activities in which strength and speed are the determining factors has been associated to the ACTN3 gene, which is responsible for the expression of α-actinin-3. Soccer is a mainly aerobic sport because of its long duration, but the acute actions that define the game demand a lot of strength and speed. The purpose of the present study was to compare the performance capacity of soccer players with different genotype groups of ACTN3 (XX, RX, and RR) in strength, speed, and endurance tests. Two hundred professional players of Brazilian soccer first division teams participated in this study. Speed, jump, and endurance test results were compared with the polymorphisms of the ACTN3 gene. It was noticed that RR individuals spent less time to run a 10-m path, compared with XX individuals (p < 0.05). The RR individuals also presented lower time rates at the 20- and 30-m path, compared with RX and XX individuals (p < 0.05). In jump tests, RR individuals presented higher rates, compared with RX and XX individuals (p < 0.05). As for aerobic tests, the XX individuals presented higher rates of V[Combining Dot Above]O2 max, compared with the RR group (p < 0.05), and did not differ from the RX group. The main conclusion of this study is that soccer players of genotype ACTN3/RR are the fastest in short distances and present higher jump potential. ACTN3/XX individuals presented the highest aerobic capacity. These findings can be used in training load adjustment and can influence the development of tactical schemes in soccer matches.

Intensity of official Futsal matches.

The purpose of this study was to assess the intensity of official Futsal matches, expressed in different ways. Fourteen male professional Futsal players from a First Division Brazilian team volunteered to participate in this study. Maximal oxygen uptake (VO(2)max) and the heart hate (HR) and oxygen uptake (VO(2)) correlation were determined for each player. The match intensity was estimated from the players' average HR measured during 13 National Futsal League matches. The HR measurements were obtained while the players were in the court but the values recorded while the players were sitting on the bench were not considered. In addition, these HR values were used to estimate the intensity of the effort expressed as a percentage of the maximal HR (% HRmax), percentage of VO(2)max (% VO(2)max), kilocalories per minute (kcal·min(-1)), and total caloric expenditure. The mean intensity of the matches was 86.4 ± 3.8% HRmax, 79.2 ± 9.0% VO(2)max, 18.0 ± 2.2 kcal·min(-1), and 313 ± 9.3 kcal. It was concluded that official Futsal matches have high intensity when expressed in the different ways used in this study. The information provided by this research can be used for planning the athletes' workouts, diets, and resting periods.